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ST 1.3 –
Efectos biológicos de las radiaciones ionizantes y Dosimetría biológica
ADVANTAGES OF MICRONUCLEI ANALYSIS THROUGH
IMAGES AUTOCAPTURING AND SCREEN SCORING
González, Jorge Ernesto
1
; Martínez-López, Wilner
2
*
1
Centro de Protección e Higiene de las Radiaciones (CPHR). Cuba.
2
Instituto de Investigaciones Biológicas Clemente Estable (IIBCE). Uruguay.
* Responsible author, email: wilner_martinez@yahoo.com
The in vitro micronucleus (MN) test is a quantitative assay for genetic toxicity assessment. One
of the advantages of the MN assay is that it is amenable for automation. Different type of cells
has been used, such as, human lymphocytes and rodent cell lines (i.e. CHO, V79, CHL and
L5178Y). The MN quantification is a time consuming process and several efforts has been
conducted for its automation. Some of them include an operator checking step, like Path Finder
Cell Scan System, or are fully automated such as MN Score from MetaSytems. Usually, fully
automated systems detect two or three times less MN than visual scoring. In some cases, the
impact of false positive detection is reduced with a visual detection step. In the present work we
have tested a combination of image autocapturing of CHOK1 cells previously treated with
bleomycin (0, 2.5, 5.0 and 10.0 µg/ml) or UVC (0, 4, 8 and 16 J/m
2
) with a screen scoring.
Autocapturing images plus screen scoring render similar results in terms of MN cells frequency
than microscopic live scoring. The resultant bias from the Bland–Altman analysis was -1.1%
with confidence intervals between -2.2% and -0.1%, indicating an acceptable agreement
between both MN scoring method. However, the mean time devoted to live microscope scoring
per sample was 159 minutes compared to 39 minutes for microscope images autocapturing and
screen scoring. Therefore, it become advantageous to combine autocapturing of microscope
images plus screen scoring when many samples have to be analyzed for radiological
biodosimetry purposes.